Protein Biochemistry and Proteomics

di Rehm • 2006 • dettagli prodotto

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DESCRIZIONE

Hubert Rehm' s Protein Biochemistry and Proteomics is more than a laboratory manual; it is a strategic guide that provides the reader with tips and tricks for more successful lab experiments. Using a conversational yet professional tone, Rehm provides an overview of a variety of methods in protein biochemistry/proteomics. He provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. Readers will immediately sense that the author if very familiar with the challenges, and frustration of the daily lab routine. Never before has such an honest, tactical guide been available for those conducting lab experiments within the field of biochemistry.ContentsThe Concept of the Experimenter Abbreviations 1. The Daily Bread 1.1 Making Buffers 1.2 Protein Determination 1.3 Gels 1.4 Staining Gels 1.5 Precipitate and Concentrating 1.6 Blotting 1.7 Autoradiography of Gels and Blots 2. Ligand Binding 2.1 Radioactive Ligand Marking 2.2 Binding 2.3 Analysis of Binding Data 2.4 Crosslinking of Ligands 2.5 Purposes 3. Solubilization of Membrane Proteins 3.1 Detergents 3.2 Solubilization 4. Protein Detection via Functional Measurements 4.1 Translocators 4.2 Reconstitution 4.3 Flux Assay 4.4 Constructive Thoughts 5. Cleaning and Purifying 5.1 Pure Fun 5.2 Conventional Purification Methods 5.3 Affinity Chromatography 5.4 The Purity Test 5.5 Profiting 6. Antibodies 6.1 Production of Polyclonal Antibodies 6.2 Immunoprecipitation 6.3 Immunoaffinity Chromatography 6.4 Antibodies Against Unpurified Proteins 6.5 Immunological detection techniques 7. Proteomics 7.1 Introduction 7.2 Sample taking 7.3 2D gel electrophoresis 7.4 Mass Spectroscopy of Peptides and Proteins 7.5 Protein Chips 7.6 Microsequencing 7.7 Strategy 8. Subunits 8.1 Number & Stoichiometry of Subunits 8.2 What Holds Our World Together 9. Glycoproteins 9.1 How, Where and for What Purpose are Proteins Glycosylated? 9.2 Detecting Glycoproteins in Gels 9.3 Detection of Glycoproteins on Blots 9.4 Deglycosylation 9.5 The Sugar Chains 10. Treasure Island 10.1 Of the Paper 10.2 Of Writing a Paper 11. Desert Planet 12. Jaws 12.1 Suppliers 12.2 Suppliers by Product