Proteases and Cancer - Methods and Protocols

di Antalis - Bugge • 2009 • dettagli prodotto

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DESCRIZIONE

Proteases decisively contribute to cancer development and promotion by regulating the activities of growth factors/cytokines and signaling receptors, as well as the composition of the extracellular matrix, thereby suppressing cell death pathways and activating cell survival pathways. In Proteases and Cancer: Methods and Protocols, expert researchers bring together a wide range of current, complimentary techniques that have been developed for the specific detection and analysis of proteases and their activities in cancer biology. The volume covers vital topics including the application of proteomics technologies for the detection of protease expression in tumors, imaging proteases by activity profiling, proteomics technologies for the identification of biological substrates, detection of cell surface proteolysis, imaging of protease activity, the use of transgenic mice to determine protease function in tumor initiation and progression, and the development of anti-protease therapies for cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Table of contents Chapter 1. Delineating Protease Functions During Cancer DevelopmentNesrine I. Affara, Pauline Andreu, and Lisa M. CoussensChapter 2. Proteolytic Systems: Constructing DegradomesGonzalo R. Ordóñez, Xose S. Puente, Víctor Quesada, and Carlos López-OtínChapter 3. Microarrays for Protease Detection in Tissues and CellsKamiar Moin, Donald Schwartz, Stephanie Mullins, and Bonnie F. SloaneChapter 4. Positional Scanning Synthetic Combinatorial Libraries for Substrate ProfilingEric L. Schneider and Charles S. CraikChapter 5. Mixture-Based Peptide Libraries for Identifying Protease Cleavage MotifsBenjamin E. TurkChapter 6. High Throughput Substrate Phage Display for Protease ProfilingBoris Ratnikov, Piotr Cieplak, and Jeffrey W. SmithChapter 7. Imaging Specific Cell Surface Protease Activity in Living Cells Using Reengineered Bacterial CytotoxinsJohn P. Hobson, Shihui Liu, Stephen H. Leppla, and Thomas H. BuggeChapter 8. Cell-Based Identification of Natural Substrates and Cleavage-Sites for Extracellular Proteases by SILAC Proteomics Magda Gioia, Leonard J. Foster, and Christopher M. OverallChapter 9. Optical Proteolytic Beacons for In Vivo Detection of Matrix Metalloproteinase ActivityJ. Oliver McIntyre and Lynn M. MatrisianChapter 10. Dissecting the Urokinase Activation Pathway Using Urokinase-Activated Anthrax ToxinShihui Liu, Thomas H. Bugge, Arthur E. Frankel, and Stephen H. LepplaChapter 11. On-Demand Cleavable Linkers for Radioimmunotherapy Pappanaicken R. Kumaresan, Juntao Luo, and Kit S. Lam